FastQCFastQC Report
Thu 2 Jun 2016
DRR015467_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015467_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14422088
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182840.12677775922598725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185100.051.159461
GTATCAA346400.041.1044581
TCAACGC412100.033.953294
ATCAACG416000.033.6568573
CAACGCA435700.032.1350755
TATCAAC452250.031.1301442
AACGCAG467700.030.109646
CGCAGAG520400.026.5501568
ACGCAGA526200.026.4768687
GTACATG476800.024.7710341
TACATGG476600.023.9257972
GCAGAGT582450.023.1271869
ACATGGG497000.022.110583
CAGAGTA575000.018.9802710-11
AGAGTAC543900.018.6812910-11
GAGTACT315950.018.25732412-13
CATGGGG277150.018.0928124
GTGGTAT91750.017.9713361
TCTGTCG32200.017.378368
GTACTTT331950.016.97974214-15
Produced by FastQC (version 0.11.5)