FastQCFastQC Report
Thu 2 Jun 2016
DRR015467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14422088
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167830.11637011228887247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169550.048.0766531
GTATCAA330850.043.5432131
TCAACGC417250.033.9604574
ATCAACG419100.033.7958033
CAACGCA434100.032.7209175
TATCAAC460100.031.0610372
AACGCAG465850.030.551016
CGCAGAG534400.026.174878
ACGCAGA539050.026.0649977
GTACATG457250.023.8490981
GCAGAGT595850.022.6277759
TACATGG458500.022.435632
ACATGGG476750.020.9464073
AGAGTAC555900.019.7682910-11
AGTACTT331550.018.30197112-13
GAGTACT319450.017.86275912-13
CAGAGTA584950.017.58856810-11
GTGGTAT83850.017.3984871
CATGGGG268350.017.199564
GTACTTT329800.016.77778214-15
Produced by FastQC (version 0.11.5)