FastQCFastQC Report
Thu 2 Jun 2016
DRR015466_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015466_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11482221
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240840.2097503610146504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188040.16376622606375543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233000.045.308571
GTATCAA418700.039.9848441
ATCAACG517400.031.9948943
TCAACGC520650.031.7602144
CAACGCA550450.029.982935
TATCAAC562150.029.6179222
AACGCAG576450.028.7860836
ACGCAGA627650.026.0246777
CGCAGAG629000.025.7423388
GTACATG611000.025.5891281
TACATGG600500.025.1121482
ACATGGG622200.023.4464933
GCAGAGT675750.023.3477349
CATGGGA322950.021.0503674
AGAGTAC615100.019.54416810-11
GTACTTT288150.019.45040514-15
CATGGGG252700.019.3574284
CAGAGTA662200.018.83075710-11
AGTACTT309050.018.14999812-13
GAGTACT320100.017.97823712-13
Produced by FastQC (version 0.11.5)