FastQCFastQC Report
Thu 2 Jun 2016
DRR015466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11482221
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219170.1908777056285539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166190.14473680658123544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222250.043.3783451
GTATCAA411000.040.780841
ATCAACG524450.031.693973
TCAACGC529000.031.4514524
CAACGCA549900.030.2406945
TATCAAC565250.029.6155512
AACGCAG572400.029.0532556
ACGCAGA645350.025.4947937
CGCAGAG644450.025.2915558
GTACATG588150.024.6768281
TACATGG580050.024.2737372
GCAGAGT692350.022.8056779
ACATGGG604450.022.4651053
CATGGGA316250.020.2976654
AGAGTAC630500.019.95840810-11
GTACTTT289250.019.12864714-15
AGTACTT310600.018.94961412-13
CATGGGG248450.018.0618744
CAGAGTA681300.017.76896910-11
GAGTACT324800.017.52575312-13
Produced by FastQC (version 0.11.5)