FastQCFastQC Report
Thu 2 Jun 2016
DRR015465_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015465_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14364715
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177480.12355274713072972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193150.048.5785331
GTATCAA358250.041.2826841
ATCAACG424950.034.244233
TCAACGC425050.034.2254644
CAACGCA445650.032.694465
TATCAAC461750.031.6234722
AACGCAG474850.030.7749826
ACGCAGA538000.026.714447
CGCAGAG539200.026.400298
GTACATG532100.025.6118051
TACATGG531250.024.5655752
GCAGAGT598700.022.9856629
ACATGGG549350.022.8119053
CATGGGG294650.018.9026094
AGAGTAC546500.018.87112410-11
CAGAGTA578450.018.75287610-11
GAGTACT292400.018.16396312-13
GTGGTAT94750.017.354011
GTACTTT304600.016.95832814-15
CATGGGA223800.016.387844
Produced by FastQC (version 0.11.5)