FastQCFastQC Report
Thu 2 Jun 2016
DRR015465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14364715
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161150.11218461347823469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176750.047.2257841
GTATCAA331700.042.536861
ATCAACG419400.033.237953
TCAACGC423100.032.9508444
CAACGCA438350.031.739445
TATCAAC450450.031.148872
AACGCAG463650.030.126846
ACGCAGA542600.025.407967
CGCAGAG543750.025.2218368
GTACATG498550.024.3873441
TACATGG501650.023.347452
GCAGAGT598450.022.0877259
ACATGGG518050.021.825863
AGAGTAC549900.019.45340210-11
GAGTACT295500.017.4921112-13
CAGAGTA589850.017.32200210-11
CATGGGG279250.017.245274
AGTACTT312500.016.69342412-13
GTACTTT306850.016.34794814-15
CATGGGA213650.016.3134824
Produced by FastQC (version 0.11.5)