FastQCFastQC Report
Thu 2 Jun 2016
DRR015464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6881193
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89340.12983213811907324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71050.10325244474322984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95500.046.354281
GTATCAA179000.041.3454061
ATCAACG211450.034.1288263
TCAACGC211150.034.0695724
CAACGCA220300.032.613215
AACGCAG232500.031.1718036
TATCAAC234300.031.0140532
ACGCAGA261800.027.2660587
CGCAGAG265050.026.7247778
GTACATG252600.026.4859391
TACATGG252000.025.6397442
ACATGGG261050.024.0536673
GCAGAGT294500.023.1093089
CATGGGG131300.019.8917524
AGAGTAC265100.019.28830710-11
CAGAGTA284100.018.59081810-11
GAGTACT146550.018.43916512-13
TACCTGG49250.018.108222
GTGGTAT50950.017.9671331
CATGGGT55650.017.4156764
Produced by FastQC (version 0.11.5)