FastQCFastQC Report
Thu 2 Jun 2016
DRR015464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6881193
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79510.11554682451138923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85750.044.9809651
GTATCAA165400.042.0365331
ATCAACG204800.033.3468133
TCAACGC205500.033.235644
CAACGCA214200.031.925035
TATCAAC226050.030.3730352
AACGCAG226450.030.3201126
ACGCAGA260650.026.0452297
CGCAGAG262150.025.6558158
GTACATG243550.025.1408791
TACATGG239450.024.3542392
ACATGGG250850.022.5029683
GCAGAGT295550.022.2021779
AGAGTAC268900.019.81644610-11
AGTACTT150450.017.75425312-13
GAGTACT145100.017.54644812-13
CATGGGG126850.017.4206964
TACCTGG53650.017.3236682
CAGAGTA286750.017.24193410-11
CATGGGA105750.017.1912214
Produced by FastQC (version 0.11.5)