FastQCFastQC Report
Thu 2 Jun 2016
DRR015463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9391922
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146920.15643230427169222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114570.1219878103757676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG103800.041.1620452
GGTATCA173400.041.0552751
GTACCTG123350.035.411031
GTATCAA286100.034.721371
TATAACG10400.033.6888432
ACCTGGG125200.033.363153
ATCAACG340150.028.7070083
TCAACGC341950.028.5558994
CAACGCA356200.027.375185
TATCAAC360650.027.1383322
AACGCAG379550.026.0240926
GTACATG363000.025.9981351
TACATGG361550.025.019392
GTACCGG10400.024.5252691
ATAACGC16050.023.5305333
ACATGGG373900.023.255943
ACGCAGA422350.022.9990627
CGCAGAG423150.022.8032328
GTATAAC26800.022.0936581
GCAGAGT454850.020.3830819
Produced by FastQC (version 0.11.5)