FastQCFastQC Report
Thu 2 Jun 2016
DRR015463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9391922
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135040.14378313618873753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99560.10600599110597383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG102700.041.74172
TATAACG10250.037.778612
GTATCAA268200.037.7381941
GGTATCA153500.037.4833071
GTACCTG118650.036.6963231
ACCTGGG123650.033.895883
ATCAACG336050.029.7837543
TCAACGC337750.029.6249334
CAACGCA348150.028.8682255
TATCAAC359850.027.9909822
AACGCAG370850.027.434336
GTACATG343400.025.1591421
TACATGG344850.024.3734532
ATAACGC17600.023.8137843
ACGCAGA430150.023.3671597
CGCAGAG432950.023.1023988
TAACGCA18450.022.9671464
ACATGGG360450.022.3834383
GTATAAC28600.021.3612021
GCAGAGT464300.020.7094219
Produced by FastQC (version 0.11.5)