FastQCFastQC Report
Thu 2 Jun 2016
DRR015462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11205590
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151780.13545025295410595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116580.10403735992482324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141750.050.1270751
GTATCAA276900.042.2253951
ATCAACG325700.035.416873
TCAACGC327700.035.2284934
CAACGCA345400.033.396865
TATCAAC355050.032.617322
AACGCAG367150.031.601096
ACGCAGA416200.027.406767
CGCAGAG415300.027.1456478
GTACATG391700.025.1171881
TACATGG392100.024.3013172
GCAGAGT460350.023.510059
TACCTGG89450.022.4849512
ACATGGG405500.022.409763
GTACCTG103100.020.2779881
AGAGTAC419750.019.17257710-11
CAGAGTA450200.018.7802110-11
GAGTACT236550.018.36622612-13
GTGGTAT70800.018.2707311
TATAACG9850.018.016832
Produced by FastQC (version 0.11.5)