FastQCFastQC Report
Thu 2 Jun 2016
DRR015462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11205590
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144690.12912305376156008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137800.045.8197631
GTATCAA268650.043.2548981
ATCAACG337800.033.8083953
TCAACGC340800.033.461364
CAACGCA353500.032.266055
TATCAAC367950.031.149272
AACGCAG374900.030.5844636
ACGCAGA437400.025.964717
CGCAGAG433150.025.937248
GTACATG371900.024.1118471
TACATGG376700.022.98862
GCAGAGT481700.022.6429169
ACATGGG392800.021.4782753
AGAGTAC443150.020.44365710-11
TACCTGG91550.018.7092132
AGTACTT263150.018.61434612-13
GAGTACT250100.017.96637712-13
GTACCTG103500.017.2662491
CAGAGTA473200.017.20778710-11
CATGGGG208850.016.8845234
Produced by FastQC (version 0.11.5)