FastQCFastQC Report
Thu 2 Jun 2016
DRR015461_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015461_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13419373
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166870.12435007209353223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178450.049.8940931
GTATCAA336350.041.7717481
ATCAACG399650.034.5445063
TCAACGC401800.034.3823134
CAACGCA422250.032.6417055
TATCAAC433750.032.0385482
AACGCAG440000.031.446556
ACGCAGA499900.027.3053267
CGCAGAG504650.026.847568
GTACATG468050.025.4836831
TACATGG470100.024.3149072
GCAGAGT558200.023.2928059
ACATGGG489450.022.6005943
GAGTACT278200.019.3450812-13
CAGAGTA541750.019.12945210-11
AGAGTAC508050.019.09994710-11
CATGGGG291400.018.6916584
GTACTTT299750.017.5897914-15
AGTACTT292600.017.15698212-13
GAGTACA363050.016.295271
Produced by FastQC (version 0.11.5)