FastQCFastQC Report
Thu 2 Jun 2016
DRR015461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13419373
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152940.11396955729600779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165150.045.3234181
GTATCAA320350.042.6329271
ATCAACG400650.033.2558253
TCAACGC402000.033.103644
CAACGCA418550.031.8392095
TATCAAC431900.031.1341442
AACGCAG438600.030.405496
ACGCAGA513300.025.6350087
CGCAGAG515350.025.3231268
GTACATG441400.024.4658781
TACATGG448600.023.0804332
GCAGAGT574000.022.0459869
ACATGGG468500.021.4196283
AGAGTAC516200.019.70138410-11
AGTACTT301550.018.05471212-13
GAGTACT285750.017.86667812-13
CAGAGTA553600.017.29365710-11
CATGGGG273500.017.2406354
GTACTTT306950.016.35831814-15
CATGGGT115100.015.63478854
Produced by FastQC (version 0.11.5)