FastQCFastQC Report
Thu 2 Jun 2016
DRR015460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12129847
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165900.13677006808082576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125460.10343081821229896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153400.047.652881
GTATCAA293150.041.2335321
TCAACGC346500.034.0497744
ATCAACG347650.033.806263
CAACGCA360350.032.6779445
TATAACG11800.031.6189042
TATCAAC380500.031.1985472
TACCTGG117650.030.9008052
AACGCAG388400.030.5857626
GTACCTG134400.027.9233461
ACGCAGA440900.026.6651527
CGCAGAG442150.026.412778
GTACATG386300.026.1031191
TACATGG383700.025.329372
ACCTGGG150150.023.4244353
GCAGAGT486700.023.3212879
ACATGGG407700.022.934333
ATAACGC18300.022.3772343
AGAGTAC450900.019.4724410-11
GTATAAC37600.019.3807981
Produced by FastQC (version 0.11.5)