FastQCFastQC Report
Thu 2 Jun 2016
DRR015460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12129847
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147030.12121340030092713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136200.045.9925961
GTATCAA271700.042.2684941
ATCAACG332850.033.8457373
TCAACGC332750.033.8191684
CAACGCA346100.032.5501025
TATCAAC361050.031.4544092
AACGCAG371600.030.4893676
TACCTGG118150.029.6496282
GTACCTG130600.027.2637831
ACGCAGA434000.025.7389097
CGCAGAG438550.025.3597228
GTACATG358550.025.1255441
TACATGG358400.023.87012
ACCTGGG145350.023.2550533
TATAACG11400.023.1766032
GCAGAGT488350.022.2145469
ACATGGG374450.021.9589613
AGAGTAC444150.020.18340710-11
AGTACTT282400.018.08784112-13
CATGGGG218650.017.523894
Produced by FastQC (version 0.11.5)