FastQCFastQC Report
Thu 2 Jun 2016
DRR015459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13605471
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162940.11976064628707085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168750.048.802481
GTATCAA311550.041.4634781
TCAACGC371050.034.17674
ATCAACG373300.034.0194663
CAACGCA389250.032.5787165
TATCAAC407450.031.2798332
AACGCAG413000.030.8894716
ACGCAGA464350.027.1404387
CGCAGAG469300.026.6879888
GTACATG430000.025.5397111
TACATGG430900.024.2132552
GCAGAGT522400.023.0341159
ACATGGG448050.022.585723
AGAGTAC481500.018.94852410-11
CAGAGTA508050.018.83136610-11
GAGTACT287650.018.39322912-13
CATGGGG262950.017.9271894
GTGGTAT82950.017.7916341
GTACTTT295950.017.65423414-15
TCTGTCG35100.017.627558
Produced by FastQC (version 0.11.5)