FastQCFastQC Report
Thu 2 Jun 2016
DRR015459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13605471
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153500.1128222609860401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151500.047.940591
GTATCAA294500.043.5496521
ATCAACG377000.033.365853
TCAACGC378500.033.1874284
CAACGCA390900.032.1464275
TATCAAC405950.031.2890622
AACGCAG411200.030.7495616
ACGCAGA480500.026.0310217
CGCAGAG484600.025.690718
GTACATG398500.023.8205971
GCAGAGT539750.022.3912759
TACATGG403950.022.387482
ACATGGG419400.020.97643
AGAGTAC494500.020.12012110-11
AGTACTT314350.017.89160312-13
CAGAGTA523550.017.39581710-11
GAGTACT299300.017.14170512-13
CATGGGG242300.016.5098784
GTACTTT310450.016.29087414-15
TATACTG70300.015.46745
Produced by FastQC (version 0.11.5)