FastQCFastQC Report
Thu 2 Jun 2016
DRR015458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7703933
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132530.17202901427102235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92820.12048391386581374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG14650.050.9384082
TACCTGG117400.048.2935452
GTACCTG128200.044.6596341
ATAACGC18350.042.4032553
GGTATCA120750.039.682271
GTATCAA219750.039.4644431
ACCTGGG139900.039.1929973
GTACCCG10050.038.5230641
ATCAACG256800.033.081753
TCAACGC256650.033.0656244
CAACGCA265350.031.9129125
GTATAAC28200.031.818891
TACCCGG11900.030.9725692
TATCAAC275850.030.8796232
TAACGCA27250.030.223954
AACGCAG293100.029.4321656
CCTGGGG91050.027.2367784
GTACATG277050.027.1429371
GTACCGG9400.027.1348931
TCTGTCG21850.026.8597098
Produced by FastQC (version 0.11.5)