FastQCFastQC Report
Thu 2 Jun 2016
DRR015458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7703933
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111420.14462742601733428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG13500.049.2688942
TACCTGG109700.047.5086982
ATAACGC16250.042.6134573
GTACCTG124900.041.979161
GTATCAA203500.039.2525631
ACCTGGG131300.037.9238133
GGTATCA110550.037.530211
ATCAACG248400.031.4901873
TCAACGC251100.031.1915234
GTATAAC25900.031.1581671
CAACGCA260000.030.1682685
GTACCCG9700.030.0819931
TATCAAC266400.029.4990882
GTACCGG7850.029.0401421
TAACGCA25050.028.738014
AACGCAG287250.027.6566076
TACCCGG10800.026.9966552
CCTGGGG87850.026.917394
TCTGTCG20600.025.844618
GTACATG259750.025.4162461
Produced by FastQC (version 0.11.5)