FastQCFastQC Report
Thu 2 Jun 2016
DRR015457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10662031
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142150.1333235665887672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110950.1040608491946797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139700.048.662641
GTATCAA259300.041.417161
TCAACGC307650.034.2278184
ATCAACG308350.034.2091453
CAACGCA323300.032.627255
TATCAAC332200.031.9038122
AACGCAG341050.031.2042436
TATAACG10600.029.1928042
ACGCAGA391450.026.710087
CGCAGAG397350.026.1501038
TACCTGG98400.025.6204912
GTACATG360800.024.019681
GTACCTG113200.022.9833281
TACATGG361150.022.894972
GCAGAGT443950.022.6659059
ACATGGG375150.021.5796173
ACCTGGG121200.019.8621313
ATAACGC13850.019.7139783
AGAGTAC397800.018.92012610-11
GTGGTAT69050.018.8723341
Produced by FastQC (version 0.11.5)