FastQCFastQC Report
Thu 2 Jun 2016
DRR015457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10662031
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132490.1242633790879055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129300.048.088051
GTATCAA247500.042.0610661
ATCAACG301150.033.9356043
TCAACGC301950.033.852224
CAACGCA312500.032.603215
TATCAAC326300.031.6273042
AACGCAG329450.031.0224846
ACGCAGA390150.025.9520117
CGCAGAG394450.025.4292188
TACCTGG95750.023.8031312
GTACATG331550.023.0602931
GCAGAGT442600.022.2205479
TACATGG335900.021.7669332
GTACCTG113100.021.1353051
ACATGGG341900.020.5583443
AGAGTAC400750.019.54991310-11
ACCTGGG118250.018.772873
TATAACG10450.017.8842352
CAGAGTA422850.017.86623810-11
GAGTACT232800.017.54546512-13
Produced by FastQC (version 0.11.5)