FastQCFastQC Report
Thu 2 Jun 2016
DRR015456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13609527
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184500.13556679817013478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143760.10563188566362373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183600.048.4041861
GTATCAA341850.041.2537771
TCAACGC404850.034.31364
ATCAACG407450.034.0834733
CAACGCA431000.032.3478285
TATCAAC440450.031.715782
AACGCAG455100.030.7428956
ACGCAGA505450.027.185377
CGCAGAG503250.027.0581158
GTACATG468000.025.228271
TACATGG465100.024.3507422
GCAGAGT565850.023.4996959
ACATGGG481800.022.7606093
AGAGTAC516350.019.45313510-11
GAGTACT295550.019.01705612-13
CATGGGG276500.018.974394
CAGAGTA562750.018.74648910-11
AGTACTT308750.017.40837512-13
GTACTTT315550.017.28539714-15
CATGGGA167900.016.3418124
Produced by FastQC (version 0.11.5)