FastQCFastQC Report
Thu 2 Jun 2016
DRR015456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13609527
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172130.12647757706788781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173600.049.043341
GTATCAA340350.042.8621441
TCAACGC416800.034.5552864
ATCAACG417950.034.4774443
CAACGCA435150.033.0658575
TATCAAC454800.031.8141862
AACGCAG458800.031.4504266
ACGCAGA525900.027.1268427
CGCAGAG524450.026.943528
GCAGAGT586500.023.4411839
GTACATG437600.022.790661
TACATGG442500.021.6788562
ACATGGG458100.020.2740943
AGAGTAC532950.020.24608810-11
GAGTACT304300.018.3128212-13
CAGAGTA578150.018.28147510-11
AGTACTT315500.018.10267612-13
GTACTTT324600.016.84377314-15
CATGGGG265600.016.2508854
CATGGGT100700.015.8866664
Produced by FastQC (version 0.11.5)