FastQCFastQC Report
Thu 2 Jun 2016
DRR015455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18610920
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270610.14540388116224237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202750.108941417189478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA250150.047.5047071
GTATCAA476200.041.9288141
TCAACGC560350.034.8935934
ATCAACG560550.034.8811453
CAACGCA599150.032.6111565
TATCAAC605050.032.4360472
AACGCAG620000.031.5769626
ACGCAGA694850.027.7367527
CGCAGAG710050.026.9486628
GTACATG662200.026.1952931
TACATGG668750.025.3251672
ACATGGG698400.023.5333213
GCAGAGT792500.023.4884079
CATGGGG477750.020.8299314
GAGTACT360550.020.28776612-13
AGAGTAC699000.019.76778810-11
CAGAGTA744550.019.2427310-11
GTACTTT398350.018.07109314-15
AGTACTT391000.017.55602312-13
GAGTACA498050.015.9826771
Produced by FastQC (version 0.11.5)