FastQCFastQC Report
Thu 2 Jun 2016
DRR015455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18610920
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256900.13803723835253712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221550.047.5515331
GTATCAA441500.042.8874051
ATCAACG543850.033.861333
TCAACGC546700.033.656794
CAACGCA567950.032.4036035
TATCAAC585500.031.693982
AACGCAG593100.031.0678336
ACGCAGA696500.026.0777637
CGCAGAG708150.025.49118
GTACATG612100.025.0305651
TACATGG618400.024.205312
ACATGGG650050.022.7041473
GCAGAGT798400.021.9654259
CATGGGG453050.020.0603924
AGAGTAC696200.019.92044310-11
GAGTACT370900.018.6407712-13
AGTACTT401100.018.08833312-13
CAGAGTA746850.017.73150310-11
TATACCG20450.016.9126855
GTACTTT406500.016.68724614-15
Produced by FastQC (version 0.11.5)