FastQCFastQC Report
Thu 2 Jun 2016
DRR015454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12953400
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205500.15864560655889573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163770.12643012645328638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182250.049.007961
GTATCAA343650.041.2270741
ATCAACG404650.034.422783
TCAACGC408300.034.1596374
CAACGCA421650.033.0673075
TATCAAC432350.032.227892
AACGCAG446250.031.6177066
ACGCAGA506950.027.5357597
CGCAGAG510000.027.1892958
TACCTGG120900.026.3471662
GTACCTG129250.025.7024841
GTACATG437250.025.012641
TACATGG434850.024.3092692
GCAGAGT562950.023.7327869
ACATGGG453300.022.4665573
ACCTGGG160000.019.339763
AGAGTAC519000.019.27410110-11
CAGAGTA549750.019.23448610-11
GAGTACT315950.018.88250212-13
TATAACG15400.018.6158122
Produced by FastQC (version 0.11.5)