FastQCFastQC Report
Thu 2 Jun 2016
DRR015454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12953400
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193780.1495977889974833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134430.1037797026263375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162400.044.1132351
GTATCAA316650.042.2224461
ATCAACG396150.033.126613
TCAACGC398000.032.9311334
CAACGCA411200.031.9939275
TATCAAC426250.030.9264132
AACGCAG434300.030.5226046
ACGCAGA505000.025.9530727
TACCTGG117700.025.762682
CGCAGAG508550.025.5507558
GTACATG403450.024.8029981
TACATGG400900.023.8852122
GTACCTG132800.023.7429811
GCAGAGT557400.022.5848149
TATAACG13750.022.2186642
ACATGGG420700.022.0673983
AGAGTAC508100.020.49001910-11
ATAACGC18700.019.2633293
ACCTGGG152700.019.2007313
TAACGCA18900.018.82074
Produced by FastQC (version 0.11.5)