FastQCFastQC Report
Thu 2 Jun 2016
DRR015453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9066061
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157440.1736586594773629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125140.13803127951598826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138200.050.8012351
GTATCAA253450.040.57131
ATCAACG307450.033.0940173
TCAACGC308900.032.923944
TACCTGG90600.031.9433962
CAACGCA320100.031.6866685
TATCAAC331950.030.8022612
AACGCAG339350.030.1942866
GTACCTG99250.029.9293121
ACGCAGA386850.026.0987057
CGCAGAG389600.025.8523858
GTACATG348550.025.7109991
ACCTGGG110200.024.6515883
TACATGG352250.024.5703682
ACATGGG357650.023.2706033
GCAGAGT420500.023.1398439
TATAACG9400.022.2680472
GTGGTAT62300.019.744921
TCTGTCG21000.019.7135168
CAGAGTA410650.019.46325910-11
Produced by FastQC (version 0.11.5)