FastQCFastQC Report
Thu 2 Jun 2016
DRR015453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9066061
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143820.15863559709117334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99980.11027942565133854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115550.048.0093351
GTATCAA226500.043.324571
TACCTGG88500.035.182932
ATCAACG294850.032.7166753
TCAACGC297350.032.4000854
CAACGCA306550.031.4861155
GTACCTG101100.031.4065461
TATAACG8250.030.944872
TATCAAC321950.030.2459642
AACGCAG329200.029.4994756
ACCTGGG107800.027.868993
ACGCAGA382900.025.1728447
GTACATG318550.025.0051041
CGCAGAG384450.024.8851178
TACATGG321900.023.7218442
ACATGGG325350.022.4474123
GCAGAGT420750.022.208119
ATAACGC14200.020.5468963
AGAGTAC390550.020.44088710-11
GTACACG8900.019.9910981
Produced by FastQC (version 0.11.5)