FastQCFastQC Report
Thu 2 Jun 2016
DRR015452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452339
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45470.13170780737349375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.10613094484637807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA130400.030.333911
GGTATCA85550.028.28531
TCAACGC147000.026.5614454
ATCAACG146750.026.5446743
GTACATG117200.025.6627141
CAACGCA152200.025.6539575
TATCAAC153300.025.5886152
AACGCAG157100.025.0522166
TACATGG117600.024.2628572
ACATGGG123500.022.4773243
ACGCAGA175750.022.1089827
CGCAGAG174400.022.0685338
GAGTACT74700.020.21700312-13
GCAGAGT185050.020.1080429
TATAACG3403.6379788E-1220.076782
GTACTTT77000.019.11069714-15
AGAGTAC132900.019.08206710-11
AGTACTT75700.017.42614712-13
CATGGGG66900.017.0057434
CATGGGC38800.016.5374724
Produced by FastQC (version 0.11.5)