FastQCFastQC Report
Thu 2 Jun 2016
DRR015452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452339
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT234340.6787861794568841No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT111130.3218977047155566RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT77620.2248330769371142RNA PCR Primer, Index 31 (95% over 24bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT46120.13359058887322478RNA PCR Primer, Index 31 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.11994766446748131No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116000.030.2117421
GGTATCA77850.025.6150571
GTACATG105100.025.0956061
TCAACGC137400.024.9560324
ATCAACG137800.024.9148713
CAACGCA140400.024.2745865
AACGCAG145100.023.7391726
TACATGG107800.023.6854782
TATCAAC146450.023.661222
ACATGGG111500.022.3270153
ACGCAGA163200.020.8284267
CGCAGAG162750.020.7486238
AGAGTAC126450.020.73051310-11
TATAACG2701.6716513E-920.2642422
GCAGAGT176450.018.750779
AGTACTT77600.018.53253712-13
TACCTGG24100.018.351332
GAGTACT72300.018.19152312-13
CATGGGA42200.017.1802354
CATGGGG62300.017.1266424
Produced by FastQC (version 0.11.5)