FastQCFastQC Report
Thu 2 Jun 2016
DRR015451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13641275
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188150.1379269899624485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150930.11064215038550282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181400.052.2019771
GTATCAA348500.042.54941
ATCAACG419250.034.8074233
TCAACGC422300.034.6314624
CAACGCA448450.032.6323285
TATCAAC454800.032.3467942
AACGCAG471300.031.171046
ACGCAGA524600.027.5617187
CGCAGAG526250.027.0746338
GTACATG506950.026.3019471
TACATGG510950.025.3634052
GCAGAGT578850.023.8497719
ACATGGG526700.023.8274173
GAGTACT283350.019.89007612-13
CAGAGTA559100.019.7660810-11
CATGGGG315200.019.5613614
AGAGTAC532950.019.38739410-11
GTGGTAT89800.018.2178361
GTACTTT303600.018.18868614-15
AGTACTT304050.017.21942712-13
Produced by FastQC (version 0.11.5)