FastQCFastQC Report
Thu 2 Jun 2016
DRR015451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13641275
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177940.13044235234609666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164850.049.8828121
GTATCAA321450.043.0996131
ATCAACG406450.033.4992873
TCAACGC410000.033.136014
CAACGCA425600.031.8488525
TATCAAC442350.030.914582
AACGCAG449600.030.1992246
ACGCAGA524650.025.5776277
CGCAGAG528150.025.2304348
GTACATG474150.025.1483041
TACATGG480250.023.9553662
ACATGGG500600.022.371263
GCAGAGT583250.022.1679639
AGAGTAC531700.019.70798710-11
CATGGGG295050.018.3935244
GAGTACT287600.018.32443412-13
CAGAGTA561050.017.92668710-11
AGTACTT309650.017.71768812-13
GTACTTT308600.016.68525714-15
GTGGTAT90000.016.174591
Produced by FastQC (version 0.11.5)