FastQCFastQC Report
Thu 2 Jun 2016
DRR015450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13559872
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206780.15249406484073005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157250.11596717137152916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176600.049.3861541
GTATCAA338050.041.462871
ATCAACG393800.034.886023
TCAACGC394700.034.875654
CAACGCA414950.033.0969165
TATCAAC427550.032.3556942
AACGCAG443250.031.2143886
CGCAGAG498550.027.2472938
ACGCAGA502950.027.2107287
GTACATG428650.024.6519871
TACATGG428200.023.751712
GCAGAGT561750.023.507669
ACATGGG438000.022.2228973
ATAACGC13750.020.84983
TATAACG12500.020.7505172
TCTGTCG34250.019.9238388
GAGTACT308550.019.48242812-13
AGAGTAC508850.019.34075410-11
GTACCTG121100.018.9644571
TACCTGG117600.018.8445452
Produced by FastQC (version 0.11.5)