FastQCFastQC Report
Thu 2 Jun 2016
DRR015450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13559872
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184830.13630659640444984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156700.045.6577261
GTATCAA308500.044.0608141
ATCAACG385800.034.862983
TCAACGC390050.034.452124
CAACGCA404700.033.215435
TATCAAC416450.032.5270352
AACGCAG432950.031.3316756
CGCAGAG498650.026.7056778
ACGCAGA503100.026.6837167
GTACATG407850.024.5232091
TACATGG402850.023.948432
GCAGAGT557950.023.1169039
ACATGGG420700.022.3470843
AGAGTAC503100.020.50795210-11
TATAACG11350.020.0853252
TACCTGG111000.019.3054352
GTACCTG120750.018.4781681
GAGTACT302950.018.14036812-13
AGTACTT331200.017.98097612-13
CATGGGG239100.017.8887234
Produced by FastQC (version 0.11.5)