FastQCFastQC Report
Thu 2 Jun 2016
DRR015449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14859122
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235530.15850869250551952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182680.12294131510596656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212500.047.7086751
GTATCAA415200.042.1295741
ATCAACG495250.034.7961353
TCAACGC494900.034.7379884
CAACGCA519050.033.095425
TATCAAC525000.033.015042
AACGCAG550300.031.4608156
ACGCAGA620400.027.5466387
CGCAGAG618500.027.4882498
GTACATG571700.025.3127081
TACATGG571100.024.5094932
GCAGAGT681250.024.272769
ACATGGG594300.022.7027843
AGAGTAC623450.019.8467110-11
GAGTACT347350.019.67713512-13
TACCTGG122500.019.093572
CATGGGG304650.018.865244
CAGAGTA673900.018.81995610-11
GTACCTG134700.018.469341
AGTACTT366300.018.23692912-13
Produced by FastQC (version 0.11.5)