FastQCFastQC Report
Thu 2 Jun 2016
DRR015449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14859122
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217790.14656989827528166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183350.046.507111
GTATCAA372900.043.7395821
ATCAACG467800.034.412853
TCAACGC469200.034.2358974
CAACGCA486850.032.9467545
TATCAAC498000.032.3442732
AACGCAG519600.031.0015096
GTACATG526850.026.4034921
CGCAGAG601400.026.3278738
ACGCAGA607500.026.247627
TACATGG527650.025.541222
ACATGGG555400.023.6083773
GCAGAGT666950.023.0851949
AGAGTAC614800.020.27973410-11
AGTACTT366300.018.6746612-13
CATGGGG291500.018.0819954
GAGTACT346100.017.91043512-13
TACCTGG122150.017.8036082
GTACCTG131000.017.3439881
CAGAGTA660550.017.3042310-11
Produced by FastQC (version 0.11.5)