FastQCFastQC Report
Thu 2 Jun 2016
DRR015448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15816340
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261510.165341665644517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206770.13073188866703675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA234550.052.013191
GTATCAA455500.041.580311
ATCAACG538850.034.557223
TCAACGC541950.034.384744
CAACGCA569600.032.691645
TATCAAC574650.032.4835322
AACGCAG599650.031.2541226
ACGCAGA687800.026.9243727
CGCAGAG685650.026.9127468
GTACATG630000.025.8387491
TACATGG634850.024.9519182
GCAGAGT761700.023.4948739
ACATGGG657450.023.1528473
TATAACG15250.020.5897142
GAGTACT379900.019.65552712-13
AGAGTAC695100.019.28332310-11
CATGGGT161100.019.1516044
CATGGGG338150.019.1364444
CAGAGTA748600.019.084110-11
TACCTGG129050.018.407022
Produced by FastQC (version 0.11.5)