FastQCFastQC Report
Thu 2 Jun 2016
DRR015448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15816340
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237560.1501990978949618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169290.10703487658965348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203400.048.1810261
GTATCAA415250.043.344111
ATCAACG528300.033.5705763
TCAACGC531350.033.3854944
CAACGCA551600.032.1828545
TATCAAC557100.031.9905912
AACGCAG587550.030.3762536
GTACATG592850.026.0807131
ACGCAGA693850.025.4945017
CGCAGAG689650.025.4874558
TACATGG594850.025.2314472
ACATGGG624300.023.3182183
GCAGAGT758750.022.4824589
CATGGGT160450.021.0604364
AGAGTAC694150.020.14562810-11
CATGGGG313450.018.7676684
GAGTACT383300.018.68410112-13
AGTACTT410350.018.6503412-13
TATAACG13800.017.8403932
CAGAGTA741950.017.33256710-11
Produced by FastQC (version 0.11.5)