FastQCFastQC Report
Thu 2 Jun 2016
DRR015447_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015447_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18049336
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201730.11176588435164594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233900.053.5145231
GTATCAA464050.042.7333871
ATCAACG548950.035.7855073
TCAACGC552400.035.5702484
CAACGCA586000.033.4763645
TATCAAC593300.033.2178652
AACGCAG622250.031.7124236
ACGCAGA691800.028.0572597
CGCAGAG691400.027.8989898
GTACATG673100.025.7042061
TACATGG669300.024.8430882
GCAGAGT764800.024.4874789
ACATGGG696400.023.2098373
GTGGTAT104600.022.5653111
CAGAGTA750900.019.79158210-11
CATGGGG381900.019.255274
AGAGTAC716300.019.0900710-11
TGGTATC114500.018.9967942
GAGTACT403650.018.93279612-13
GTACTTT407250.018.24027414-15
Produced by FastQC (version 0.11.5)