FastQCFastQC Report
Thu 2 Jun 2016
DRR015447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18049336
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189900.10521162662161089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213350.049.2965471
GTATCAA422650.042.8487741
ATCAACG527700.033.9567643
TCAACGC530450.033.698984
CAACGCA554450.032.2871635
TATCAAC571950.031.3934082
AACGCAG594350.030.2114476
CGCAGAG679350.025.9262168
ACGCAGA686050.025.8959147
GTACATG630150.025.5315131
TACATGG636650.024.514722
ACATGGG659950.022.9237753
GCAGAGT755950.022.606659
AGAGTAC706450.019.71002410-11
GTGGTAT101000.019.2905541
CATGGGG360750.018.1898694
CAGAGTA747900.017.61664410-11
GAGTACT395100.017.43385912-13
AGTACTT411550.017.38946312-13
CATGGGA240150.016.8238834
Produced by FastQC (version 0.11.5)