FastQCFastQC Report
Thu 2 Jun 2016
DRR015446_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015446_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17152203
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283880.16550643669504145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229430.13376124338080653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA265150.050.678221
GTATCAA503200.043.8853151
TCAACGC623250.035.0451974
ATCAACG626050.034.8666533
CAACGCA657300.033.2159085
TATCAAC663050.032.969042
AACGCAG690000.031.7769226
ACGCAGA770650.028.0440317
CGCAGAG774450.027.7154318
GTACATG698250.024.9270021
GCAGAGT846050.024.4930639
TACATGG704350.023.6645362
ACATGGG729000.022.2838523
GAGTACT412100.020.38948212-13
AGAGTAC772550.020.01591110-11
CAGAGTA816650.019.86813410-11
GTGGTAT118750.019.2745361
CATGGGG455350.018.8571534
GTACTTT440450.018.82365614-15
AGTACTT429050.018.49715812-13
Produced by FastQC (version 0.11.5)