FastQCFastQC Report
Thu 2 Jun 2016
DRR015446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17152203
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260020.15159568715458885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196460.11453922274590617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA241100.047.9695131
GTATCAA462750.043.7449151
TCAACGC605900.033.0319754
ATCAACG606750.032.998993
CAACGCA629450.031.7283385
TATCAAC643150.031.2093372
AACGCAG664600.030.1529646
ACGCAGA771800.025.6996757
CGCAGAG769800.025.6006248
GTACATG648200.024.2121851
TACATGG657100.023.0956632
GCAGAGT844950.022.6557039
ACATGGG682200.021.5442523
AGAGTAC771400.020.4171610-11
AGTACTT440750.019.13481312-13
GAGTACT422950.018.80482712-13
CAGAGTA816100.018.00780510-11
CATGGGG422200.017.9250684
GTGGTAT117250.017.6655651
GTACTTT454700.017.14925214-15
Produced by FastQC (version 0.11.5)