FastQCFastQC Report
Thu 2 Jun 2016
DRR015445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20065933
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318810.15888122421220086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254900.12703122252027851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA275250.052.1400871
GTATCAA530150.041.9107441
ATCAACG625050.034.9651373
TCAACGC627050.034.868134
CAACGCA664100.032.9228445
TATCAAC676650.032.406362
AACGCAG700300.031.3411666
CGCAGAG773050.027.8653378
ACGCAGA783250.027.673457
GTACATG641750.024.2279421
GCAGAGT861950.024.1622289
TACATGG644750.023.2330322
ACATGGG665400.021.9307563
GAGTACT493100.020.56883812-13
GTGGTAT131400.019.7386251
CAGAGTA851500.019.67973510-11
AGAGTAC797050.019.65145310-11
GTACTTT540950.018.50107214-15
AGTACTT522250.017.85281812-13
CATGGGG362850.017.4812744
Produced by FastQC (version 0.11.5)