FastQCFastQC Report
Thu 2 Jun 2016
DRR015445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20065933
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283000.14103505678006598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210340.10482443054105682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246550.048.795821
GTATCAA489100.043.588871
ATCAACG615850.034.1128233
TCAACGC620000.033.8494954
CAACGCA646300.032.458515
TATCAAC664750.031.7337422
AACGCAG682600.030.8390626
CGCAGAG783100.026.3749358
ACGCAGA792550.026.2454457
GTACATG599950.023.4138131
GCAGAGT877750.022.9033819
TACATGG610300.022.1194952
ACATGGG621100.020.9494483
AGAGTAC807100.020.42781410-11
AGTACTT537750.018.6879312-13
GAGTACT504250.018.41309412-13
CAGAGTA863750.017.77597810-11
GTGGTAT129300.017.0422171
GTACTTT555350.016.50721214-15
TCTGTCG48250.016.3189758
Produced by FastQC (version 0.11.5)