FastQCFastQC Report
Thu 2 Jun 2016
DRR015444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13989822
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213050.1522892857393039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155190.1109306465800637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168650.046.6773831
GTATCAA341000.042.8482131
ATCAACG394600.036.313023
TCAACGC395150.036.3085374
TACCTGG146100.036.1288872
CAACGCA414150.034.6318175
TATCAAC425650.033.6533932
GTACCTG161050.033.5944751
AACGCAG452950.032.1136326
TATAACG15600.029.169042
CGCAGAG505200.028.2853368
ACCTGGG181300.028.210793
ACGCAGA510150.028.1561837
GTACATG413100.025.9407481
ATAACGC19000.025.3862783
TACATGG415600.024.897842
GCAGAGT558000.024.4993529
ACATGGG434900.022.8617153
TCTGTCG37850.020.9141438
AGAGTAC509200.020.2270410-11
Produced by FastQC (version 0.11.5)