FastQCFastQC Report
Thu 2 Jun 2016
DRR015444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13989822
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197570.14122409849103157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150700.044.7472841
GTATCAA307750.043.0382351
TACCTGG142600.034.9439052
ATCAACG388150.033.6508373
TCAACGC389550.033.5681344
GTACCTG152400.033.4106331
TATAACG15950.033.1564562
CAACGCA408650.032.0255555
TATCAAC421150.031.0248852
AACGCAG444950.029.7605576
ACCTGGG178300.027.2569123
ATAACGC20950.026.9841333
CGCAGAG513650.025.3510118
ACGCAGA518100.025.3479927
GTACATG386700.024.4231471
TACATGG388750.023.5368182
GCAGAGT562850.022.49629
ACATGGG412250.021.5205293
AGAGTAC516550.020.4064310-11
GTATAAC41850.019.296711
Produced by FastQC (version 0.11.5)