FastQCFastQC Report
Thu 2 Jun 2016
DRR015443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15329724
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236150.15404713092029576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181200.1182017367044573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208950.049.585771
GTATCAA398050.041.3015861
ATCAACG476750.033.9587483
TCAACGC477850.033.947234
CAACGCA499250.032.5285645
TATCAAC515250.031.4566382
AACGCAG530300.030.843636
ACGCAGA593800.027.0472287
CGCAGAG591800.026.9133998
GTACATG501150.025.0053311
TACATGG499250.024.243762
GCAGAGT647250.023.6514269
ACATGGG518400.022.610873
AGAGTAC593650.019.49151610-11
GAGTACT358100.019.38612412-13
CAGAGTA641050.019.21768410-11
CATGGGG292100.019.207374
GTACTTT375300.018.10955614-15
GTGGTAT104050.017.6237931
AGTACTT371450.017.07886312-13
Produced by FastQC (version 0.11.5)