FastQCFastQC Report
Thu 2 Jun 2016
DRR015443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15329724
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211000.13764109516909762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189150.043.729811
GTATCAA364750.041.7644961
ATCAACG473850.031.614693
TCAACGC475100.031.5625694
CAACGCA493150.030.3939295
TATCAAC510250.029.6006132
AACGCAG524350.028.7329486
ACGCAGA603000.024.6237057
CGCAGAG600400.024.5307488
GTACATG459950.023.8357751
TACATGG459600.022.8442132
GCAGAGT666000.021.3559429
ACATGGG479500.021.0404513
AGAGTAC604850.020.34700610-11
AGTACTT392500.018.12440312-13
CAGAGTA656850.017.3296749
GAGTACT374350.017.03960812-13
CATGGGG273050.015.9127124
GTACTTT393750.015.90321114-15
TACCTGG109450.015.6198822
Produced by FastQC (version 0.11.5)